Request transgenic mice from the Richardson/Kessaris labs

back to Richardson home page Kessaris home page last updated 31 Aug 2010

Request transgenic mice from the Bill Richardson/ Nicoletta Kessaris labs

We thank the UK Medical Research Council, The Wellcome Trust, the European Research Council, the Biotechnology and Biological Sciences Research Council, the US National Institutes of Health and The Royal Society for past and present support.

We have generated a number of transgenic strains, mainly BAC or PAC transgenics by pronuclear injection. If you wish to obtain these please follow the steps below.

email Bill Richardson and/or Nicoletta Kessaris (see table below) outlining your proposed project in a few sentences.

Please note that our animal facility is currently testing positive for Mouse Hepatitis Virus (MHV) which could create a problem for your animal facility. Please first check with your animal facility that they can receive the mice before we initiate the export process. The latest Health Reports for our animals can be found here: Health report Mar 08, Health report Oct 08, MHV screen Jan 09, Health report May 09 room 1, Health report May 09 room 2, Health report June 10 room 1, Health report Nov 11 room 1, Health report July 12 room 1, Health report Feb 13 room 1. Further queries about mouse health status or husbandry should be directed to Nick Davies. Here are answers to some Frequently Asked Questions.

Complete the mouse request form and email it to our transgenic facility manager Ulla Dennehy

Download the relevant Material Transfer Agreement (MTA) from the table below, sign and return direct to UCL Business (address is on the MTA).

For shipments outside the UK please download the Home Office Transfer Form and complete/amend the highlighted sections. Then email it to Ulla Dennehy for signature (you do not need to sign). We try to ship mice as soon as they are ready (usually within 3-4 months) but please allow a time window of 1 year for the shipment on the HO transfer form.

For shipments within the UK, please download the UK Home Office Transfer Form, complete/amend the highlighted sections and return by post to Ulla Dennehy, Wolfson Institute for Biomedical Research, UCL, Gower Street, London WC1E 6BT. Alternatively, you might already have permission written into your Project Licence to import/export animals within the UK, in which case you do not need to complete the transfer form – but please let Ulla Dennehy know.

We normally ship three (3) males of breeding age. We regret that we cannot routinely ship embryos.

Please note that we depend on you to pay costs. Our facility routinely uses Harlan UK for the shipment– unfortunately, we cannot use other couriers. Shipment is organized by Nick Davies

The following mice are available:

Strain	Animal code	Contact	Vector	Made by	References	MTA or JAX Stock	Genotyping protocol	Comments/ expression pattern
Dbx1-iCre	H142	Kessaris cc Richardson	PAC	Fogarty/Kessaris	1, 4, 5	Dbx1-iCre	Dbx1-iCre or iCre	Cortico-striatal boundary, spinal cord p1, p2, dP6, dP5
Dlx1-lox-Venus-lox	A666	Kessaris	BAC	Rubin/Alfonsi/ Kessaris	Unpublished	Dlx1-Venus	Dlx-Venus	Forebrain
Emx1-iCre	H151	Kessaris cc Richardson	PAC	Fogarty/Kessaris	3, (suppl info), 4, 5	Emx1-iCre	Emx1-iCre or iCre or use UV lamp at P0-P1*	Cortical neuroepithelium
Emx1-CreER^T2	G199	Kessaris cc Richardson	PAC	Fogarty/Kessaris	3, 5	Emx1-CreER^T2 27784	Emx1-CreER^T2	Cortical neuroepithelium
Fgfr3-iCreER^T2	K121	Kessaris cc Richardson	PAC	Mitsumori/Kessaris	7 (suppl info), 13 (suppl info 1, 2)	Fgfr3-iCreER^T2 25809	iCre	Neuroepithelial cells, SVZ cells, protoplasmic and fibrous astrocytes
Gsh2-iCre	H153	Kessaris cc Richardson	PAC	Kessaris	3, (suppl info), 4, 5, 10, 12	Gsh2-iCre 25806	Gsh2-iCre or iCre	LGE/MGE/CGE
Lhx6-iCre	N104	Kessaris	BAC	Kessaris	4, 6	Lhx6-iCre 26555	iCre or use UV lamp at early stages*	MGE
Msx3-iCre	K133	Kessaris cc Richardson	PAC	Fogarty/Kessaris	Unpublished	Msx3-iCre	Msx3-iCre or iCre or use UV lamp at early stages*	Dorsal spinal cord dP1, dP2, dP3, dP4 and neural crest
Nkx2.2-CreER^T2	H101	Kessaris cc Richardson	PAC	Taveira-Marques/Kessaris	Unpublished	Nkx2.2-CreER^T2	Nkx2.2-CreER^T2	Ventral spinal cord p3
Nkx2.1-iCre	G178	Kessaris cc Richardson	PAC	Kessaris	3, (suppl. info), 4, 10, 12	Nkx2.1-iCre	Nkx2.1-iCre or iCre	MGE
Nkx2.1-iCre-ER^T2	K185	Kessaris cc Richardson	PAC	Kessaris	Unpublished	Nkx2.1-iCre-ER^T2 25808	Nkx2.1-iCre or iCre	MGE
Nkx6.2-iCre	L129	Kessaris	BAC	Kessaris	4	Nkx6.2-iCre 27798	iCre	LGE/MGE boundary, spinal cord
Olig2-Cre	E193	Richardson	Knock-in	Rowitch/Kessaris	3, (suppl. info), 14	Olig2-Cre 25567	Olig2-Cre	Spinal cord pMN, oligodendrocyte lineage
Olig2-NLS-eGFP	H152	Richardson	PAC	Callard/Kessaris	Unpublished	Olig2-NLS-eGFP	Olig2-GFP	Spinal cord pMN, oligodendrocyte lineage
Pdgfra-CreER^T2	H126	Richardson	PAC	Jamen/Kessaris	7 (suppl info), 15	Pdgfra-CreER^T2	Pdgfra-CreER^T2	Oligodendrocyte progenitors postnatally (ectopic dorsal expression in embryo)
Sox1-iCreER^T2	K184	Kessaris cc Richardson	BAC	Kessaris	Unpublished	Sox1-iCreER^T2	iCre	Embryonic neuroepithelium and adult neural stem cells
Sox10-Cre	F155	Kessaris cc Richardson	PAC	Iannarelli/Kessaris	2, 8, 9, 11	Sox10-Cre 25807	Sox10-Cre or use UV lamp at early stages*	Oligodendrocyte lineage, neural crest, Schwann cells
Sox10-lox-GFP-STOP-lox-DTA	K152	Kessaris cc Richardson	PAC	Iannarelli/Kessaris	3, (suppl. info)	Sox10- GFP-DTA	Sox10-DTA or use UV lamp at early stages	Oligodendrocyte lineage, neural crest, Schwann cells
Zic4-iCre	A611	Kessaris	BAC	Kessaris/Rubin	Unpublished	Zic4-iCre	iCre	Septal telencephalic and diencephalic regions

* A UV lamp can be used to genotype these mice when a Cre reporter allele expressing a fluorescent protein is also present