Paul R Barber Principal Research Fellow
Molecular Oncology Group
UCL Cancer Institute
Department of Oncology
University College London

ORCID iD iconorcid.org/0000-0002-8595-1141

I am a scientific programmer and advanced microscopy expert with over 18 years’ experience working on and leading multi-disciplinary biomedical projects.



Research Interests

Vessel Tracing

Fluorescence Lifetime Imaging (FLIM) detection and data processing for Fluorescence Resonance Energy Transfer (FRET) measurements of key protein dimer interactions in cells, tissues and exosomes. The use of machine learning to form multivariate models that can be used to form risk signatures and discover groups of patients who did respond to treatment in otherwise failed clinical trials. Automated microscopy, image analysis and processing for high-content microscopy and digital pathology.


Current Highlights

In collaboration with Tony Ng and his group and Ton Coolen I am working to combine FLIM/FRET measurements of dimer interactions from patient samples with immune cell signatures and clinical data to predict patient outcome. Machine learning helps us to stratify patients for a personalised response to treatment.

In collaboration with Simon Ameer-Beg and Simon Poland we are developing the next generation of FLIM systems, called SWARM, to speed up dimer interaction profiling in patient samples and to move from single regions of interest to whole slide imaging.

In collaboration with Kevin Eliceiri and Curtis Reuden, UW Madison, we are working to make SLIM Curve a valuable open source project for lifetime fitting of FLIM data. It is already core to TRI2 and the SWARM acquisition pipeline.

Technical Skills

Neural Network Segmentation

Microscopy, Imaging, Computer Programming, Laser physics, Instrument control, C (LabWindows/CVI, Glib, GTK+), Python, R, GStreamer, TWiki, Computing, Analysis, Image and signal processing and algorithm development and coding.

Links

My Twitter
My Github
Molecular Oncology Group - Tony Ng
Advanced Technology Development Group at Oxford - Boris Vojnovic
ATDGroup Github
Vojnovic Group at Dept of Oncology
CyMap - Winner of The Engineer Award 2009
TRI2 web site
SLIM Curve web site

VIDEO: Me talking about the CyMap for Marblar.

My Instructables page: programming the Raspberry Pi and ATtiny microprocessor.

Contact Me
p.barber at ucl.ac.uk