@Article {Shellard339, author = {Shellard, Adam and Szab{\'o}, Andr{\'a}s and Trepat, Xavier and Mayor, Roberto}, title = {Supracellular contraction at the rear of neural crest cell groups drives collective chemotaxis}, volume = {362}, number = {6412}, pages = {339--343}, year = {2018}, doi = {10.1126/science.aau3301}, publisher = {American Association for the Advancement of Science}, issn = {0036-8075}, URL = {http://science.sciencemag.org/content/362/6412/339}, eprint = {http://science.sciencemag.org/content/362/6412/339.full.pdf}, journal = {Science} } @Article{Roycroft2018, author = {Roycroft, Alice AND Szabó, András AND Bahm, Isabel AND Daly, Liam AND Charras, Guillaume AND Parsons, Maddy AND Mayor, Roberto}, journal = {Developmental Cell }, title = {Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest}, year = {2018}, volume = {45}, number = {5}, pages = {565 - 579.e3}, issn = {1534-5807}, doi = {https://doi.org/10.1016/j.devcel.2018.05.003}, url = {https://www.sciencedirect.com/science/article/pii/S1534580718303605} } @Article{Szabo2017, author = {Szab\'{o}, And\'{a}s AND Merks, Roeland M. H.}, journal = {PLOS Computational Biology}, publisher = {Public Library of Science}, title = {Blood vessel tortuosity selects against evolution of aggressive tumor cells in confined tissue environments: A modeling approach}, year = {2017}, month = {07}, volume = {13}, url = {https://doi.org/10.1371/journal.pcbi.1005635}, pages = {1-32}, number = {7}, doi = {10.1371/journal.pcbi.1005635} } @Article{Szabo2016c, author = {Szab\'{o}, Andr\'{a}s and Melchionda, Manuela and Nastasi, Giancarlo and Woods, Mae L and Campo, Salvatore and Perris, Roberto and Mayor, Roberto}, title = {In vivo confinement promotes collective migration of neural crest cells}, journaL = {Journal of Cell Biology}, volume = {213}, year = {2016}, number = {5}, pages = {542-555}, url = {http://jcb.rupress.org/content/213/5/543.abstract}, doi = {10.1083/jcb.201602083}, } @Article{Szabo2016b, author = {Szab\'{o}, Andr\'{a}s and Cobo, Isidoro and Omara, Sharif and McLachlan, Sophie and Keller, Ray and Mayor, Roberto}, title = {The molecular basis of radial intercalation during tissue spreading in early development}, journaL = {Developmental Cell}, volume = {37}, year = {2016}, number = {3}, pages = {213-225}, url = {http://www.cell.com/developmental-cell/fulltext/S1534-5807%2816%2930203-9}, doi = {10.1016/j.devcel.2016.04.008}, } @Article{Szabo2016a, author = {Szab\'{o}, Andr\'{a}s and Mayor, Roberto}, title = {Modelling collective cell migration of neural crest}, journaL = {Current Opinion in Cell Biology}, volume = {42}, year = {2016}, number = {}, pages = {22-28}, url = {http://dx.doi.org/10.1016/j.ceb.2016.03.023}, doi = {10.1016/j.ceb.2016.03.023}, } @Article{Scarpa2015, author = {Scarpa, Elena and Szab\'{o}, Andr\'{a}s and Bibonne, Anne and Theveneau, Eric and Parsons, Maddy and Mayor, Roberto}, title = {Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces}, journaL = {Developmental Cell}, volume = {34}, year = {2015}, number = {4}, url = {http://dx.doi.org/10.1016/j.devcel.2015.06.012}, doi = {10.1016/j.devcel.2015.06.012}, } @Article{Szabo2015, author = {András Szabó and Roberto Mayor}, title = {Cell traction in collective cell migration and morphogenesis: The chase and run mechanism}, journal = {Cell Adhesion \& Migration}, volume = {9}, number = {5}, pages = {380-383}, year = {2015}, doi = {10.1080/19336918.2015.1019997}, note ={PMID: 26267782}, URL = {http://dx.doi.org/10.1080/19336918.2015.1019997}, eprint = {http://dx.doi.org/10.1080/19336918.2015.1019997} } @Article{Palachanis2015, author = {Dimitris Palachanis and Szab\'{o}, Andr\'{a}s and Merks, Roeland M H}, title = {Particle-based simulation of ellipse-shaped particle aggregation as a model for vascular network formation}, journaL = {Computational Particle Mechanics}, volume = {2}, year = {2015}, number = {4}, url = {http://link.springer.com/article/10.1007%2Fs40571-015-0064-5}, doi = {10.1007/s40571-015-0064-5}, issn = {2196-4386} } @article{Czirok2013, author={András Czirók and Katalin Varga and Előd Méhes and András Szabó}, title={Collective cell streams in epithelial monolayers depend on cell adhesion}, journal={New Journal of Physics}, volume={15}, number={7}, pages={075006}, url={http://stacks.iop.org/1367-2630/15/i=7/a=075006}, year={2013}, } @Article{Szabo2013, author = {Szab\'{o}, Andr\'{a}s and Merks, Roeland M H}, title = {Cellular Potts modeling of tumor growth, tumor invasion and tumor evolution}, journaL = {Frontiers in Oncology}, volume = {3}, year = {2013}, number = {87}, url = {http://www.frontiersin.org/molecular_and_cellular_oncology/10.3389/fonc.2013.00087/abstract}, doi = {10.3389/fonc.2013.00087}, issn = {2234-943X} } @Article{Lagendijk2013, title = {Hyaluronan: A critical regulator of endothelial-to-mesenchymal transition during cardiac valve formation}, journal = {Trends in Cardiovascular Medicine}, volume = {-}, number = {doi: 10.1016/j.tcm.2012.10.002}, pages = {-}, year = {2013}, issn = {1050-1738}, doi = {10.1016/j.tcm.2012.10.002}, url = {http://www.sciencedirect.com/science/article/pii/S105017381200401X", author = {Lagendijk, Anne Karine and Szab\'{o}, Andr\'{a}s and Merks, Roeland M.H. and Bakkers, Jeroen} } @Article{Szabo2008, title = {{Multicellular sprouting in vitro.}}, author = {Szabo, Andras and Mehes, Elod and Kosa, Edina and Czirok, Andras}, abstract = {Cell motility and its guidance through cell-cell contacts is instrumental in vasculogenesis and in other developmental or pathological processes as well. During vasculogenesis, multicellular sprouts invade rapidly into avascular areas, eventually creating a polygonal pattern. Sprout elongation, in turn, depends on a continuous supply of endothelial cells, streaming along the sprout toward its tip. As long-term videomicroscopy of in vitro cell cultures reveal, cell lines such as C6 gliomas or 3T3 fibroblasts form multicellular linear arrangements in vitro, similar to the multicellular vasculogenic sprouts. We show evidence that close contact with elongated cells enhances and guides cell motility. To model the patterning process we augmented the widely used cellular Potts model with an inherently nonequilibrium interaction whereby surfaces of elongated cells become more preferred adhesion substrates than surfaces of well-spread, isotropic cells.}, journal = {Biophysical Journal}, month = sep, number = {6}, pages = {2702--2710}, volume = {95}, year = {2008}, doi = {10.1529/biophysj.108.129668}, pmid = {18567632}, issn = {0006-3495}, url = {http://www.sciencedirect.com/science/article/pii/S0006349508784157} } @Article{Szabo2007, title = {{Network Formation of Tissue Cells via Preferential Attraction to Elongated Structures}}, author = {Szabo, Andras and Perryn, Erica and Czirok, Andras}, journal = {Physical Review Letters}, month = jan, number = {3}, volume = {98}, year = {2007}, url = {http://link.aps.org/doi/10.1103/PhysRevLett.98.038102}, pages = {038102}, doi = {10.1103/PhysRevLett.98.038102} } @Article{Szabo2012, title = {{Invasion from a cell aggregate-the roles of active cell motion and mechanical equilibrium.}}, author = {Szab\'{o}, A and Varga, K and Garay, T and Heged\"us, B and Czir\'{o}k, A}, journal = {Physical Biology}, month = feb, number = {1}, pages = {016010}, volume = {9}, year = {2012}, pmid = {22313673}, doi = {10.1088/1478-3975/9/1/016010}, issn = {1478-3975}, url = {http://stacks.iop.org/1478-3975/9/i=1/a=016010}, abstract = {Cell invasion from an aggregate into a surrounding extracellular matrix (ECM) is an important process during development disease, e.g., vascular network assembly or tumor progression. To describe the behavior emerging from autonomous cell motility, cell-cell adhesion and contact guidance by ECM filaments, we propose a suitably modified cellular Potts model. We consider an active cell motility process in which internal polarity is governed by a positive feedback from cell displacements, a mechanism that can result in highly persistent motion when constrained by an oriented ECM structure. The model allows us to explore the interplay between haptotaxis, matrix degradation and active cell movement. We show that for certain conditions the cells are able to both invade the ECM and follow the ECM tracks. Furthermore, we argue that enforcing mechanical equilibrium within a bulk cell mass is of key importance in multicellular simulations.} } @Article{Szabo2011, title = {{Extracellular matrix fluctuations during early embryogenesis.}}, author = {Szab\'{o}, A and Rupp, P a and Rongish, B J and Little, C D and Czir\'{o}k, A}, journal = {Physical Biology}, month = jul, number = {4}, pages = {045006}, volume = {8}, year = {2011}, pmid = {21750366}, abstract = {Extracellular matrix (ECM) movements and rearrangements were studied in avian embryos during early stages of development. We show that the ECM moves as a composite material, whereby distinct molecular components as well as spatially separated layers exhibit similar displacements. Using scanning wide field and confocal microscopy we show that the velocity field of ECM displacement is smooth in space and that ECM movements are correlated even at locations separated by several hundred micrometers. Velocity vectors, however, strongly fluctuate in time. The autocorrelation time of the velocity fluctuations is less than a minute. Suppression of the fluctuations yields a persistent movement pattern that is shared among embryos at equivalent stages of development. The high resolution of the velocity fields allows a detailed spatio-temporal characterization of important morphogenetic processes, especially tissue dynamics surrounding the embryonic organizer (Hensen's node).}, doi = {10.1088/1478-3975/8/4/045006}, issn = {1478-3975}, url = {http://stacks.iop.org/1478-3975/8/i=4/a=045006} } @Article{Szabo2010, title = {{The Role of Cell-Cell Adhesion in the Formation of Multicellular Sprouts}}, author = {Szab\'{o}, Andr\'{a}s and Czir\'{o}k, Andr\'{a}s}, abstract = {Collective cell motility and its guidance via cell-cell contacts is instrumental in several morphogenetic and pathological processes such as vasculogenesis or tumor growth. Multicellular sprout elongation, one of the simplest cases of collective motility, depends on a continuous supply of cells streaming along the sprout towards its tip. The phenomenon is often explained as leader cells pulling the rest of the sprout forward via cell-cell adhesion. Building on an empirically demonstrated analogy between surface tension and cell-cell adhesion, we demonstrate that such a mechanism is unable to recruit cells to the sprout. Moreover, the expansion of such hypothetical sprouts is limited by a form of the Plateau-Taylor instability. In contrast, actively moving cells – guided by cell-cell contacts – can readily populate and expand linear sprouts. We argue that preferential attraction to the surfaces of elongated cells can provide a generic mechanism, shared by several cell types, for multicellular sprout formation.}, doi = {10.1051/mmnp/20105105.The}, journal = {Mathematical Modelling of Natural Phenomena}, number = {1}, pages = {106--122}, volume = {5}, year = {2010}, url = {http://www.mmnp-journal.org/action/displayAbstract?fromPage=online&aid=8023849} } @Article{Aleksandrova2012, title = {{Convective tissue movements play a major role in avian endocardial morphogenesis.}}, author = {Aleksandrova, Anastasiia and Czir\'{o}k, Andr\'{a}s and Szab\'{o}, Andr\'{a}s and Filla, Michael B and Hossain, M Julius and Whelan, Paul F and Lansford, Rusty and Rongish, Brenda J}, abstract = {Endocardial cells play a critical role in cardiac development and function, forming the innermost layer of the early (tubular) heart, separated from the myocardium by extracellular matrix (ECM). However, knowledge is limited regarding the interactions of cardiac progenitors and surrounding ECM during dramatic tissue rearrangements and concomitant cellular repositioning events that underlie endocardial morphogenesis. By analyzing the movements of immunolabeled ECM components (fibronectin, fibrillin-2) and TIE1 positive endocardial progenitors in time-lapse recordings of quail embryonic development, we demonstrate that the transformation of the primary heart field within the anterior lateral plate mesoderm (LPM) into a tubular heart involves the precise co-movement of primordial endocardial cells with the surrounding ECM. Thus, the ECM of the tubular heart contains filaments that were associated with the anterior LPM at earlier developmental stages. Moreover, endocardial cells exhibit surprisingly little directed active motility, that is, sustained directed movements relative to the surrounding ECM microenvironment. These findings point to the importance of large-scale tissue movements that convect cells to the appropriate positions during cardiac organogenesis.}, issn = {1095-564X}, journal = {Developmental Biology}, month = jan, number = {2}, pages = {348--361}, volume = {363}, year = {2012}, pmid = {22280991}, doi = {10.1016/j.ydbio.2011.12.036}, publisher = {Elsevier Inc.}, url = {http://www.ncbi.nlm.nih.gov/pubmed/22280991} } @Article{Szabo2010a, title = {{Collective cell motion in endothelial monolayers.}}, author = {Szab\'{o}, A and \:{U}nnep, R and M\'{e}hes, E and Twal, W O and Argraves, W S and Cao, Y and Czir\'{o}k, A}, abstract = {Collective cell motility is an important aspect of several developmental and pathophysiological processes. Despite its importance, the mechanisms that allow cells to be both motile and adhere to one another are poorly understood. In this study we establish statistical properties of the random streaming behavior of endothelial monolayer cultures. To understand the reported empirical findings, we expand the widely used cellular Potts model to include active cell motility. For spontaneous directed motility we assume a positive feedback between cell displacements and cell polarity. The resulting model is studied with computer simulations and is shown to exhibit behavior compatible with experimental findings. In particular, in monolayer cultures both the speed and persistence of cell motion decreases, transient cell chains move together as groups and velocity correlations extend over several cell diameters. As active cell motility is ubiquitous both in vitro and in vivo, our model is expected to be a generally applicable representation of cellular behavior.}, journal = {Physical Biology}, month = jan, number = {4}, pages = {046007}, volume = {7}, year = {2010}, url = {http://stacks.iop.org/1478-3975/7/i=4/a=046007}, doi = {10.1088/1478-3975/7/4/046007}, pmid = {21076204}, issn = {1478-3975} } @Article{Lagendijk2013, title = {{Hyaluronan: A critical regulator of endothelial-to-mesenchymal transition during cardiac valve formation.}}, author = {Lagendijk, Anne Karine and Szab\'{o}, Andr\'{a}s and Merks, Roeland M H and Bakkers, Jeroen}, abstract = {During embryonic development, cardiac valves arise at specific regions in the cardiac endothelium that swell up due to enhanced extracellular matrix production (so-called endocardial cushions). An important extracellular matrix component that is produced by the endocardial cells is the glycosaminoglycan hyaluronan. A deficiency in hyaluronan synthesis results in a failure to form endocardial cushions and a loss of their cellularization by a process called endothelial-to-mesenchymal transformation. Expression of the major hyaluronan synthase Has2 is under the influence of both positive and negative regulators. MicroRNA-dependent degradation of Has2 is required to control extracellular hyaluronan levels and thereby the size of the endocardial cushions. In this article, we review the current literature on hyaluronan synthesis during cardiac valve formation and propose that a balanced activity of both positive and negative regulators is required to maintain the critical homeostasis of hyaluronan levels in the extracellular matrix and thereby the size of the endocardial cushions. The activating and inhibitory interactions between microRNA-23, Has2, and hyaluronan are reminiscent of a reaction-diffusion system. Using a mathematical modeling approach we show that the system can produce a confined expression of hyaluronan, but only if the inhibitory signal is transferred to adjacent cells in exosomes.}, journal = {Trends in Cardiovascular Medicine}, month = jan, year = {2013}, url = {http://www.sciencedirect.com/science/article/pii/S105017381200401X}, doi = {10.1016/j.tcm.2012.10.002}, issn = {1050-1738}, pmid = {23295082} }